Sparvoli F et al. 1994
- Authors:
Sparvoli F. Martin C. Scienza A. Gavazzi G. Tonelli C.
- Title:
Cloning and molecular analysis of structural genes involved in flavonoid
and stilbene biosynthesis in grape (Vitis vinifera L.).
- Reference location:
Plant Molecular Biology. 24(5):743-55, 1994 Mar.
- Abstract:
Genes involved in flavonoid and stilbene biosynthesis were isolated from
grape (Vitis vinifera L.). Clones coding for phenylalanine ammonia-lyase
(PAL), chalcone synthase (CHS), chalcone isomerase (CHI), flavanone
3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin
dioxygenase (LDOX) and UDP glucose:flavonoid 3-O-glucosyl transferase
(UFGT), were isolated by screening a cDNA library, obtained from mRNA from
seedlings grown in light for 48 h using snapdragon (Antirrhinum majus) and
maize heterologous probes. A cDNA clone coding for stilbene synthase
(StSy) was isolated by probing the library with a specific
oligonucleotide. These clones were sequenced and when the putative
products were compared to the published amino acid sequence for
corresponding enzymes, the percentages of similarity ranged from 65%
(UFGT) to 90% (CHS and PAL). The analysis of the genomic organization and
expression of these genes in response to light shows that PAL and StSy
genes belong to large multigene families, while the others are present in
one to four copies per haploid genome. The steady-state level of mRNAs
encoded by the flavonoid biosynthetic genes as determined in young
seedlings is coordinately induced by light, except for PAL and StSy, which
appear to be constitutively expressed.
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