TITLE>Schmid GH et al. 1975</TITLE>
<H1>Schmidt GH et al. 1975</H1>
<UL>
<LI><B>Authors:</B>
  Schmid GH.  Radunz A.  Menke W.
<LI><B>Title:</B>
  The effect of an antiserum to plastocyanin on various chloroplast
  preparations.
<LI><B>Reference location:</B>
  Zeitschrift fur Naturforschung - Section C - Biosciences.  30(2):201-12,
  1975 Mar-Apr.
<LI><B>Abstract:</B>
  A monospecific antiserum to tobacco plastocyanin agglutinates stroma-free
  swellable chloroplasts from wild type tobacco, (Nicotiana tobacum var.
  John William's Broadleaf) from the tobacco aurea mutant Su/su2, (Nicotiana
  tabacum var. Su/su2) from <A HREF="../snapdragon.html">Antirrhinum majus</A> and spinach (Spinacia
  oleracea). In this condition the antiserum inhibits linear photosynthetic
  electron flow in tobacco and spinach chloroplasts. This inhibition of
  electron transport as well as the agglutination are not observed if the
  chloroplasts have been sonicated prior to antiserum addition. This is due
  to the fact that plastocyanin is removed by ultasonication. The antiserum
  stimulates a number of photophosphorylation reactions in tobacco
  chloroplasts. This stimulation is always larger in the aurea mutant
  chloroplasts from yellow leaf patches of a variegated tobacco mutant (N.
  tabacum, var. NC 95) than in the green type chloroplasts. The stimulation
  appears to be a consequence of the inhibition of linear electron
  transport. The antiserum does not affect PMS-mediated cyclic
  photophosphorylation in tobacco chloroplasts from the wild type whereas
  the reaction appears stimulated in the tobacco mutant chloroplasts.
  However, menadione-mediated cyclic photophosphorylation is inhibited upon
  addition of the antiserum. The same is true for noncyclic
  photophosphorylation coupled to electron transport in the aerobic system
  diaminodurene/ascorbate leads to methylviologen in the presence of
  N-tetraphenyl-p-phenylenediamine in spinach chlorplasts. If the lamellar
  system of <A HREF="../snapdragon.html">Antirrhinum</A> and spinach has lost is swellability neither
  agglutination nor inhibition of electron transport is observed. However,
  also in this state antibodies to plastocyanin are specifically adsorbed
  onto the surface to the thylakoid membrane. This state which is
  characterized by a morphologically well preserved lamellar system is
  realized in chloroplast preparations from <A HREF="../snapdragon.html">Antirrhinum</A> and spinach and is
  termed stroma-freed chloroplasts. In both states of the molecular
  structure of the thylakoid membrane, plastocyanin is located in the outer
  surface of the thylakoid. However, it cannot be excluded that functioning
  plastocyanin is also located in the interior of the thylakoid membrane.

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