Sablowski RW et al. 1994
- Authors:
Sablowski RW. Moyano E. Culianez-Macia FA. Schuch W. Martin C. Bevan
M.
- Title:
A flower-specific Myb protein activates transcription of phenylpropanoid
biosynthetic genes.
- Reference location:
EMBO Journal. 13(1):128-37, 1994 Jan 1.
- Abstract:
Synthesis of flavonoid pigments in flowers requires the co-ordinated
expression of genes encoding enzymes in th phenylpropanoid biosynthetic
pathway. Some cis-elements involved in the transcriptional control of
these genes have been defined. We report binding of petal-specific
activities from tobacco and Antirrhinum majus (snapdragon) to an element
conserved in promoters of phenylpropanoid biosynthetic genes and
implicated in expression in flowers. These binding activities were
inhibited by antibodies raised against Myb305, a flower-specific Myb
protein previously cloned from Antirrhinum by sequence homology. Myb305
bound to the same element and formed a DNA-protein complex with the same
mobility as the Antirrhinum petal protein in electrophoretic mobility
shift experiments. Myb305 activated expression from its binding site in
yeast and in tobacco protoplasts. In protoplasts, activation also required
a G-box-like element, suggesting co-operation with other elements and
factors. The results strongly suggest a role for Myb305-related proteins
in the activation of phenylpropanoid biosynthetic genes in flowers. This
is consistent with the genetically demonstrated role of plant Myb proteins
in the regulation of genes involved in flavonoid synthesis.
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