- Authors:
Radunz A.
- Title:
On a quantitative determination of antibodies to lipids and proteins.
- Reference location:
Zeitschrift fur Naturforschung - Section C - Biosciences.
38(3-4):297-301, 1983 Mar-Apr.
- Abstract:
The amount of precipitating antibodies in monospecific lipid- and protein
antisera was determined by quantitative precipitation reactions according
to the methods of Heidelberger and Kendall. Antisera were obtained by
immunization of rabbits. Fragments of the thylakoid membrane from
Antirrhinum chloroplasts were used as antigens for the binding of
antibodies. These fragments had a diameter of 100 A. They were composed of
51% proteins and 39% lipids. It was found that antisera to the lipids
mono-, tri- and digalactosyl diglyceride, sulfoquinovosyl diglyceride and
phosphatidyl glycerol contained 20 to 92 micrograms precipitating
antibodies per ml serum. In antisera to the coupling factor of
photophosphorylation, cytochrome f, a polypeptide with the apparent MW
24000 and a serum to the total protein of the lamellar system 23 to 99
micrograms were found. In chloroplast antisera the amount of all
precipitating lipid- and protein antibodies amounted to 765 micrograms per
ml. The described antisera originated from blood that had been withdrawn
from the test animals 4 to 10 week after antigen injection.
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