Radunz A 1976
- Authors:
Radunz A.
- Title:
Localization of the tri- and digalactosyl diglyceride in the thylakoid
membrane with serological methods.
- Reference location:
Zeitschrift fur Naturforschung - Section C - Biosciences.
31(9-10):589-93, 1976 Sep-Oct.
- Abstract:
Trigalactosyl diglyceride was isolated from leaves of Urtica dioica and
characterized by thin layer chromatography, infrared spectroscopy and by
its fatty acid composition. An antiserum to the trigalactolipid was
obtained by immunization of rabbits. By means of inhibition experiments
with oligosaccharides and mono- and digalactosyl glycerol it was
demonstrated that the antibodies are directed towards the
alpha-galactosyl-(1 leads to 6)-alpha-galactosyl-(1 leads to
6)-beta-galactosyl-(1 leads to 1)-glycerol configuration of the
trigalactosyl diglyceride. Monogalactosyl diglyceride and sulfoquinovosyl
diglyceride do not react with this antiserum. However, a cross reaction
was observed with digalactosyl diglyceride. The presence of antibodies to
tri- and digalactosyl diglyceride was demonstrated in antisera to
different chloroplast preparations of Antirrhinum majus and Spinacia
oleracea. The antiserum to the trigalactolipid agglutinates stroma-freed
chloroplasts. Membrane fragments obtained by the ultra sonication were
precipitated. The antiserum is exhausted by trigalactosyl diglyceride but
not by digalactosyl diglyceride or digalactosyl glycerol. The antiserum
treated with digalactosyl glycerol and digalactosyl diglyceride also
agglutinated stroma-freed chloroplasts. 1 g stroma-freed chloroplasts
binds 0.17 g antibodies to trigalactolipid. Membrane fragments bind more
antibodies to trigalactolipids than stroma-freed chloroplasts. From the
agglutination tests it follows that the antigenic determinants of the
trigalactolipid and the digalactolipid are localized in the outer surface
as well as in the surface directed towards the inside of the thylakoid
membrane.
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