O'Brochta DA 1994

• Authors: O'Brochta DA. Warren WD. Saville KJ. Atkinson PW.
• Title: Interplasmid transposition of Drosophila hobo elements in non-drosophilid insects.
• Reference location: Molecular & General Genetics. 244(1):9-14, 1994 Jul 8.
• Abstract: A modified hobo element from Drosophila melanogaster was introduced into embryos of the housefly, Musca domestica (family Muscidae) and the Queensland fruitfly, Bactrocera tryoni (family Tephritidae) to assess its ability to transpose. Hobo was capable of transposition in these species and transposition products had all of the hallmarks of hobo transposition products recovered from D. melanogaster, including the movement only of sequences precisely delimited by the inverted terminal repeats of hobo, the creation of an 8 bp duplication of the insertion site and an absolute requirement for hobo-encoded transposase. Transposition of hobo into the target gene resulted in a non-random distribution of insertion sites, with 10 of 38 independent insertions into the same nucleotide position. The results indicate that hobo can transpose in heterologous species, further demonstrating the similarity of hobo to Ac (Activator) of Zea mays and Tam3 of Antirrhinum majus. Hobo has excellent potential to act as a gene vector or gene tagging agent in nondrosophilid insects.

  ---

  This page is part of the Snapdragon Home Page.
  The URL of the Snapdragon Home Page is http://www.mpiz-koeln.mpg.de/snapdragon/snapdragon.html
  If you have any comments, additions or corrections to this page of general interest you are invited to use the Snapdragon Guest Book. For personal comments please write to the author(s) of this page or to Kurt Stueber.

  ---

  This page has last been modified on May 26, 1997.