Hudson AD et al. 1990
- Authors:
Hudson AD. Carpenter R. Coen ES.
- Title:
Phenotypic effects of short-range and aberrant transposition in
Antirrhinum majus.
- Reference location:
Plant Molecular Biology. 14(5):835-44, 1990 May.
- Abstract:
We describe two novel ways in which changes in gene expression in
Antirrhinum majus may arise as a consequence of the Tam3 transposition
mechanism. One involves excision of Tam3 from the nivea gene promoter and
insertion of two new Tam3 copies 3.4 kb and 2.1 kb away, on either side of
the excision site. One of the new insertions is in the nivea coding region
and completely blocks production of an active gene product. This allele
probably arose by a symmetrical double transposition, following chromosome
replication. The second case involves a small deletion at one end of Tam3
in the pallida gene, flanked by a sequence typical of a Tam3 excision
footprint. This suggests that the end of Tam3 was cleaved at an early step
in an attempted transposition and re-ligated back to its original flanking
sequence. The alteration restores some expression to the pallida gene,
suggesting that the ends of the intact Tam3 element contain components
which can actively inhibit gene expression. The implications of these
findings for the mechanism of Tam3 transposition and for the effects of
Tam3 on host gene expression are discussed.
This page is part of the
Snapdragon Home Page.
The URL of the Snapdragon Home Page is
http://www.mpiz-koeln.mpg.de/snapdragon/snapdragon.html
If you have any comments, additions or corrections to this page of general
interest you are invited to use the
Snapdragon Guest Book.
For personal comments please write to the author(s) of this page or to
Kurt Stueber.
This page has last been modified on May 26, 1997.