Green PB et al. 1990

• Authors: Green PB, Linstead P
• Title: Aprocedure for SEM of complex shoot structures applied to the inflorescence of snapdragon (Antirrhinum )
• Location: Protoplasma 158:33-38 (1990)
• Abstract: An indirect procedure for the scanning electron microscopy of living complex shoot structures, e.g., an inflorescence tip, employs the polymerization of a dental impression plastic. Application of plastic to exposed surfaces during pr olonged dissection minimizes desication. The resulting complex mould is everted so that recesses representing surface detail can be filled with molten epoxy polymer. The mould is then allowed to revert to its original configuration; it is now filled with epoxy. After the epoxy hardens, the resulting cast is sputter-coated and imaged. Intricate Structures up to 1mm in dimension can be imaged with all components, e.g., flowers on an inflorescence, in situ.