Godwin i; Todd G; Ford-lloyd b; Newbury Hj 1991
- Authors: Godwin i; Todd G; Ford-lloyd b; Newbury Hj
- Title: The effects of acetosyringone and ph on agrobacterium mediated
transformation vary according to plant species
- Location: Plant cell reports, 9 (12). 1991. 671-675.
- Abstract: explants of five plant species (allium cepa, Antirrhinum majus,
brassica campestris, glycine max, and nicotiana tabacum) were
co-cultivated with three agrobacterium tumefaciens strains under
different conditions to assess the effects of acetosyringone and
medium ph on strain virulence. tumours were incited on all
dicotyledonous species by strains n2/73 and a281. the presence of
acetorsyringone during co-cultivation generally enhanced the
virulence of these strains, most markedly n2/73 on a. majus and g.
max, and a281 on g. max. strain ach5 was virulent only on n.
tabacum in the absence of acetosyringone, which, when present,
extended the host range to include a. majus. there was evidence to
suggest that acetosyringone may suppress virulence in some
strain/plant species interactions. virulence was affected in some
cases by medium ph, but there was no general effect across plant
species.
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