Garcia-Maroto F et al. 1993
- Authors:
Garcia-Maroto F. Salamini F. Rohde W.
- Title:
Molecular cloning and expression patterns of three alleles of the
Deficiens-homologous gene St-Deficiens from Solanum tuberosum.
- Reference location:
Plant Journal. 4(5):771-80, 1993 Nov.
- Abstract:
Three alleles of the Deficiens-homologous potato gene St-Deficiens
(St-Def) present in the genome of a tetraploid Solanum tuberosum variety
were isolated and characterized. For one allele (St-Def pD13) the complete
molecular structure was determined by sequence analysis and comparison
with its cDNA, while for the other two alleles (pD10, pD12) only partial
sequences of regulatory and coding regions were obtained. All three
alleles showed (except for one amino acid exchange in pD10) identical
sequences in the coding region. While sequence variation was observed
within the respective promoters starting some 300 nt upstream of the
putative transcriptional start site, the 3' terminal promoter sequences
were highly conserved. Within this region, a sequence of 81 nt was
identified which showed 73% sequence identity to a corresponding sequence
in the Deficiens promoter. This region which contains a putative serum
response element was previously shown to regulate the expression of the
Deficiens gene in Antirrhinum majus. Expression patterns for the three
alleles in transgenic potato lines expressing St-Def promoter/Gus
constructs were identical. GUS activity was predominantly located in
petals and stamens as expected for the activity of a Def-homologous gene,
but a significant level of expression was also detected in the ovary wall
and in the vascular bundles supporting anthers and petals. The promoters
were also active in abscission structures at the junction of flower stem
and pedicel, as well as in anther stomium.
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